Figure 2.

Tumour aggression is reduced by knockdown MIR210HG (a) MIR210HG knockdown was achieved in Panc-1 and MIA PaCa-2 cells by transfecting vectors containing short hairpin RNA targeting MIR210HG (sh1/2-MIR210HG); sh-NC was transfected as a negative control. The transfection efficiency was confirmed by RT-qPCR. (b) Then, Panc-1 and MIA PaCa-2 cells were transfected with sh1/2-MIR210HG or sh-NC and examined for cell viability by CCK-8 assay; (c) DNA synthesis capacity (scale bar, 50 µm); (d) cell invasion by Transwell assay; (e) cell migration by Wound healing assay; (f) the cellular protein levels of ki67 and PCNA in no transfected cells (control) and sh1/2-MIR210HG or sh-NC transfected cells were determined by Immunoblotting. (g-h) xenograft mouse tumour model was established in BALB/C nude mice accordingly. sh-MIR210HG lentivirus infected Panc-1 cells were injected under the skin of the left flank of nude mice. Tumour size was measured every 3 days for 27 days; on day 27, mice were sacrificed and the tumour weight was measured (G-H); (i) the protein levels of ki67 and PCNA in xenograft tumour tissues were examined using Immunoblotting. *P< 0.05, **P< 0.01, compared to sh-NC. ## P< 0.01, compared to control