Figure 3.

MIR210HG modulates glycolysis-related HK2 and PKM2 and pancreatic cancer cell glycolysis (a) Four datasets, GSE55643, GSE16515, GSE15471 andGSE28735, were used to select differentially expressed genes between normal pancreatic and cancer tissues (|logFC|> 0.56, p < 0.05) and a total of 403 genes were obtained. These differentially expressed genes were applied for KEGG signalling annotation. The differentially expressed genes such as PKM2, ENO2, HK1, and HK2 were enriched in hsa00010 Glycolysis/Gluconeogenesis-Homo sapiens (human). (b) HK2 and PKM2 expression in normal pancreatic and cancer tissues, according to GSE55643. HK2: logFC = 2.037, p-value = 1.56E-05; PKM2: logFC = 1.264, p-value = 2.10E-04 (c) HK2 and PKM2 expression in normal pancreatic and cancer tissues, according to GSE16515. HK2: logFC = 0.166, p-value = 4.62E-02; PKM2: logFC = 1.757, p-value = 2.52E-08. (d) HK2 and PKM2 expression in normal pancreatic and cancer tissues, according to GDC-TCGA-PAAD and GTEx. HK2: logFC = 3.106, p-value = 3.67E-65; PKM2: logFC = 5.110, p-value = 4.51E-38. (e) The expression of HK2 and PKM2 was examined in 20 paired pancreatic cancer and adjacent non-cancerous tissue samples using RT-qPCR. (f) Panc-1 and MIA PaCa-2 cells were transfected with or without sh-MIR210HG and examined for the expression of HK2 and PKM2 using RT-qPCR. (g) Panc-1 and MIA PaCa-2 cells were transfected with or without sh-MIR210HG and examined for the protein levels of HK2 and PKM2 using Immunoblotting. Then, Panc-1 and MIA PaCa-2 cells were transfected with sh-MIR210HG and examined for glucose levels (h), lactic acid levels (i), ATP levels (j), and PDH activity (k). *P< 0.05, **P< 0.01, compared to normal or adjacent tissues group, or sh-NC group. ## P< 0.01, compared to control