Figure 6.

MIR210HG acts on pancreatic cancer cell through miR-125b-5p (a-b) Panc-1 and MIA PaCa-2 cells were co-transfected with sh-MIR210HG and miR-125b-5p inhibitor and examined for the mRNA expression and protein levels of HK2, PKM2, ki67, and PCNA by RT-qPCR and Immunoblotting. Then, Panc-1 and MIA PaCa-2 cells were co-transfected with sh-MIR210HG and miR-125b-5p inhibitor and examined for cell viability by CCK-8 assay (c); DNA synthesis capacity (scale bar, 50 µm) (d); cell invasion by Transwell assay (E); cell migration by wound healing assay (F); glucose levels (G), lactic acid levels (H), ATP levels (I), and PDH activity (J). *P< 0.05, **P< 0.01, compared to sh-NC +inhibitor NC. ##P< 0.01, compared to sh-MIR210HG+miR-125b-5p inhibitor