Table 11.
The relative mRNA and protein expression of HK2, PKM2, ki67, and PCNA in response to knockdown of MIR210HG and miR-125b-5p
| mRNA level | Sh-NC + inhibitor NC | Sh-MIR210HG + inhibitor NC | Sh-NC + miR-125b-5p inhibitor | Sh-MIR210HG + miR-125b-5p inhibitor |
|---|---|---|---|---|
| PANC-1 HK2 |
1.001 ± 0.040 | 0.529 ± 0.025**## | 1.568 ± 0.082**## | 1.031 ± 0.045 |
| MIAPaCa-2 HK2 |
1.001 ± 0.043 | 0.572 ± 0.023**## | 1.513 ± 0.040**## | 0.967 ± 0.082 |
| PANC-1 PKM2 |
1.000 ± 0.020 | 0.514 ± 0.019**## | 1.567 ± 0.071**## | 1.033 ± 0.035 |
| MIA PaCa-2 PKM2 |
1.001 ± 0.046 | 0.476 ± 0.026**## | 1.447 ± 0.017**## | 0.987 ± 0.098 |
| PANC-1 Ki-67 |
1.002 ± 0.057 | 0.308 ± 0.019**## | 1.945 ± 0.186**## | 1.158 ± 0.049 |
| MIA PaCa-2 Ki-67 |
1.004 ± 0.087 | 0.565 ± 0.051**## | 1.807 ± 0.179**## | 1.141 ± 0.087 |
| PANC-1 PCNA |
1.003 ± 0.076 | 0.456 ± 0.050**## | 1.929 ± 0.158**## | 1.161 ± 0.189 |
| MIA PaCa-2 PCNA |
1.001 ± 0.037 |
0.521 ± 0.050**## |
2.120 ± 0.078**## |
1.205 ± 0.102 |
| Protein level |
Sh-NC + inhibitor NC |
Sh-MIR210HG + inhibito NC |
Sh-NC + miR-125b-5p inhibitor |
Sh-MIR210HG + miR-125b-5p inhibitor |
| PANC-1 HK2 |
1 ± 0.105 | 0.21 ± 0.029 **## | 2.416 ± 0.213**## | 0.972 ± 0.059 |
| MIA PaCa-2 HK2 |
1 ± 0.086 | 0.25 ± 0.032 **## | 2.162 ± 0.182**## | 0.989 ± 0.049 |
| PANC-1 PKM2 |
1 ± 0.094 | 0.51 ± 0.053 **## | 1.877 ± 0.136**## | 1.211 ± 0.082 |
| MIA PaCa-2 PKM2 |
1 ± 0.058 | 0.23 ± 0.061 **## | 2.413 ± 0.154**## | 1.058 ± 0.073 |
| PANC-1 Ki-67 |
1 ± 0.073 | 0.187 ± 0.034**## | 1.877 ± 0.163**## | 0.891 ± 0.066 |
| MIA PaCa-2 Ki-67 |
1 ± 0.064 | 0.261 ± 0.031**## | 2.782 ± 0.213**## | 0.913 ± 0.068 |
| PANC-1 PCNA |
1 ± 0.036 | 0.531 ± 0.042**## | 1.582 ± 0.171**## | 0.975 ± 0.084 |
| MIA PaCa-2 PCNA |
1 ± 0.048 | 0.219 ± 0.031**## | 2.392 ± 0.136**## | 0.988 ± 0.103 |
*P < 0.05, **P < 0.01, compared to mimics Sh-NC + inhibitor NC group; #P < 0.05, ##P < 0.01, compared to Sh-MIR210HG + miR-125b-5p inhibitor group, n = 3.