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. 2021 Nov 11;12:772859. doi: 10.3389/fimmu.2021.772859

Figure 4.

Figure 4

Thrombin Mediates Platelet Aggregation and NET Release within the Lung Vasculature during IAV Infection. (Ai) Representative IVM images of thrombin probe cleavage in the mouse lung 5d-post IAV-infection at the time of probe infusion (0 min, left) and 1 min after probe infusion (right; neutrophils, red; thrombin activity, green). (Aii) Quantification of thrombin activity measured as a % of the FOV labelled by the fluorescent substrate in IAV-infected control and argatroban-pretreated animals. Quantification of neutrophils (B), platelet aggregation (C), extracellular histone (D), BALF cellularity (E), lung MPO activity (F), lung wet-to-dry tissue weight ratios (G), animal body mass (H) and viral loads (I) in IAV-infected control and argatroban-pretreated mice. Values represent the mean +/- SEM. n=4-8 per group. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the control group. #p < 0.05, ##p < 0.01 compared to the IAV-infected group.