Table 3.

DNA damage caused by bacterial infection and intracellular survival in healthy (hTERT-HPNE), early (Capan-2) and late-stage differentiation cancer (AsPC-1) cell lines

Sample ID	Isolate	hTERT-HPNE			Capan-2			AsPC-1
		%pH2A.X+	%Cell death	%Intracellular survival	%pH2A.X+	%Cell death	%Intracellular survival	%pH2A.X+	%Cell death	%Intracellular survival
L1	K. aerogenes	36.70 ± 5.51	45.40 ± 3.32***	0.92 ± 0.17	74.77 ± 8.63*	52.27 ± 1.16***	0.20 ± 0.06	33.07 ± 7.52	45.57 ± 1.96*	0.48 ± 0.12
L2	E. cloacae	45.17 ± 8.80*	38.00 ± 11.27*	0.01 ± 0.002	66.77 ± 7.67*	87.00 ± 1.61***	0.01 ± 0.01	15.91 ± 10.23	72.43 ± 4.33***	0.01 ± 0.01
	E. faecalis	N.D.	N.D.	4.65 ± 5.31	N.D.	N.D.	3.13 ± 2.63	N.D.	N.D.	0.39 ± 0.004
H1	G. adiacens	37.77 ± 7.64*	26.27 ± 5.28*	0.05 ± 0.02	54.40 ± 3.75*	32.43 ± 4.41	0.08 ± 0.05	37.67 ± 2.85***	25.97 ± 2.21	0.40 ± 0.50
	K. oxytoca	6.95 ± 1.32	38.07 ± 2.11****	0.003 ± 0.002	44.07 ± 3.56**	54.37 ± 5.60**	0.01 ± 0.001	25.37 ± 7.01*	68.30 ± 3.80***	0.004 ± 0.004
	C. freundii	2.85 ± 0.36	8.93 ± 1.14	0.02 ± 0.01	37.30 ± 1.93*	17.43 ± 2.43	0.02 ± 0.01	9.15 ± 2.92	43.97 ± 10.15*	0.05 ± 0.003
H2	E. cloacae	N.D.	N.D.	13.55 ± 2.08	N.D.	N.D.	2.05 ± 0.11	N.D.	N.D.	9.28 ± 0.69
	S. anginosus (milleri) group	3.49 ± 0.83**	12.87 ± 1.60*	1.55 ± 0.42	14.37 ± 3.16	25.40 ± 1.14	0.21 ± 0.06	1.59 ± 0.27	13.17 ± 3.96	0.29 ± 0.02
	E. faecium	3.35 ± 1.73*	11.32 ± 2.59	6.32 ± 0.57	13.57 ± 1.55	31.97 ± 1.99	8.08 ± 2.19	1.98 ± 0.03	10.88 ± 3.16	1.95 ± 0.14
C1	E. faecalis	52.77 ± 27.33	8.97 ± 1.56	0.54 ± 0.06	69.80 ± 4.06*	31.67 ± 10.53	1.10 ± 0.75	65.37 ± 10.17*	35.70 ± 8.51	0.20 ± 0.03
	S. maltophilia	15.07 ± 1.72*	9.00 ± 0.76*	0.59 ± 0.07	25.40 ± 6.42	18.43 ± 2.23	0.73 ± 0.15	10.30 ± 1.13	19.30 ± 7.72	1.16 ± 0.26
C2	E. cloacae	50.37 ± 6.11**	55.63 ± 4.73***	1.56 ± 0.28	29.87 ± 1.45**	57.03 ± 3.10**	0.13 ± 0.13	80.20 ± 5.55**	51.03 ± 4.36*	0.26 ± 0.09
	K. pneumoniae	49.50 ± 9.34*	28.73 ± 0.74**	3.63 ± 0.17	23.03 ± 3.69**	54.53 ± 1.06**	0.46 ± 0.16	53.87 ± 4.78**	39.73 ± 5.42*	0.23 ± 0.04
	S. anginosus (milleri) group	7.50 ± 0.98	17.10 ± 4.89	0.11 ± 0.04	34.17 ± 3.96	33.33 ± 4.89	0.27 ± 0.15	9.25 ± 0.45	25.90 ± 1.87	0.18 ± 0.06
	Uninfected	11.41 ± 8.06	9.69 ± 3.37		36.28 ± 6.98	23.31 ± 5.33		15.79 ± 13.22	16.72 ± 8.92

Results are shown as mean ± SD. Statistical significance was determined using unpaired t-test with Welch’s correction. Results significantly different than the uninfected control are listed in bold (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).