Figure 5.

BPLF1 expression is associated with the formation of smaller SQSTM1/p62 aggregates. (A) Endogenous SQSTM1/p62 was detected by immunofluorescence in HeLa cells transfected with either empty vector, BPLF1 or BPLF1[C61A]. Scale bar: 10 μm. (B) Box and whiskers plot of the size of SQSTM1/p62 dots. A decrease in the median size of the SQSTM1/p62 dots was observed in the presence of catalytically active BPLF1. Median size: vector and BPLF1[C61A] = 0.140 μm; BPLF1 = 0.1 μm. (C) Small SQSTM1/p62 dots were overrepresented and large dots underrepresented in cells expressing the active viral enzyme. Figures D and E were constructed by pooling data from nine independent experiments