Figure 9.

The SQSTM1/p62[E409A,K420R] mutant rescues the accumulation of HTTQ109-GFP aggregates induced by BPLF1. (A) HeLa cells were co-transfected with plasmids expressing HTTQ109-GFP, FLAG-BPLF1 and increasing amount of HA-SQSTM1/p62[E409A,K420R] and cell lysates collected after 48 h were analyzed in filter trap assays and immunoblotting. Representative immunoblots illustrating the capacity of HA-SQSTM1/p62[E409A,K420R] to rescue the accumulation of HTTQ109-GFP aggregates in a dose dependent manner. (B) Quantification of data presented in A; means ± SEM of three independent experiments. (C) Representative immunoblots illustrating the failure to rescue the accumulation of HTTQ109-GFP aggregates upon expression of SQSTM1/p62[K7A]. (D) Quantification of data presented in C; means ± SEM of three independent experiments. Statistical analysis was performed using Student t-test. *P ≤ 0.05