Figure 4.

The function of CUS-induced MDSCs. Purified MDSCs isolated from 4T1 mice (4T1-MDSC) or CUS + 4T1 mice (CUS-MDSC) were cocultured with carboxyfluorescein succinimidyl ester-labeled splenic cells at a ratio of 2:1 or 1:1 in the presence of anti-CD3/anti-28 Dynabeads for 72 h. The proliferation of CD3⁺ T cells was analyzed by flow cytometry. (a) Representative data from a single experiment, (b) Mean ± SEM from three independent experiments. (c) IFN-γ in the supernatants was detected by ELISA. Data represent mean ± SEM from three independent experiments. **P < .01, Mann-Whitney test. NS, no significant difference. (d-e) 4T1-MDSCs or CUS+4T1-MDSCs were grown in the lower compartment of transwell chambers. 4T1 cells were seeded in the upper compartment of the same chambers. The cells were incubated at 37°C for 48 h, and then, 4T1 cells that migrated to the lower surface were determined by crystal violet staining. (d). Representative images from a single experiment (e). Mean ± SEM from three independent experiments. **P < .01, Mann-Whitney test. NS, no significant difference