Table 1.
Antibodies used
| ATG2A | ab226931 | Abcam | 1/500 | WB | |
|---|---|---|---|---|---|
| ATG16L1 | 8089 | D6D5 | Cell Signaling Technology | 1/1000 | WB |
| ACTB/β-actin | A2066 | Sigma | 1/10,000 | WB | |
| B4GALT1/β4-GALT | HPA010807 | Sigma | 1/200 | IF | |
| EEA1 | 2411 | Cell Signaling | 1/500; 1/1000 | IF WB | |
| EGFR | sc-03 | 1005 | Santa Cruz Biotechnology | 1/400; 1/1000 | IF WB |
| LAMP1 | H4A3 | H4A3 | USBiological Life Sciences | 1/500; 1/1000 | IF WB |
| MAP1LC3B/LC3 | NB100-2220 | Novus Biologicals | 1/1000 | WB | |
| SNX1 | 611,482 | 51/SNX1 | BD transduction Laboratories | 1/500 | IF |
| SNX6 | HPA049374 | Atlas antibodies | 1/200 | IF | |
| TFRC | 13–6800 | H68.4 | Invitrogen | 1/200 | IF |
| WIPI1 | H00055062-M02 | 3 C1 | Abnova | 1/1000 | WB |
| WIPI1 | W2394 | Sigma | 1/1000 | WB | |
| WIPI2 | HPA019852 | Sigma | 1/500 | WB | |
| WDR45B/WIPI3 | sc-514,194 | B-7 | Santa Cruz | 1/500 | WB |
| WDR45/WIPI4 | ab240905 | Abcam | 1/1000 | WB | |
| Cy3-conjugated AffiniPure donkey anti-mouse IgG (H + L) secondary antibody | 715–165-151 | Jackson Immuno Research | 1/400 | IF | |
| Cy3-conjugated AffiniPure donkey anti-rabbit IgG (H + L) secondary antibody | 711–165,152 | Jackson Immuno Research | 1/400 | IF | |
| Alexa Fluor® 488-conjugated AffiniPure donkey anti-mouse IgG (H + L) secondary antibody | 715–545-151 | Jackson Immuno Research | 1/400 | IF | |
| Alexa Fluor® 488-conjugated AffiniPure donkey anti-rabbit IgG (H + L) secondary antibody | 711–545-152 | Jackson Immuno Research | 1/400 | IF | |
| Alexa Fluor® 647-conjugated AffiniPure donkey anti-rabbit IgG (H + L) secondary antibody | 711–605-152 | Jackson Immuno Research | 1/400 | IF | |
| Alexa Fluor® 647-conjugated AffiniPure donkey anti-mouse IgG (H + L) secondary antibody | 715–605-151 | Jackson Immuno Research | 1/400 | IF | |
| IRDye® 800CW goat anti-mouse IgG (H + L) | 926–32,210 | Li-Cor | 1/10,000 | WB | |
| IRDye® 800CW goat anti-rabbit IgG (H + L) | 926–32,211 | Li-Cor | 1/10,000 | WB | |
| IRDye® 680RD goat anti-mouse IgG (H + L) | 926–68,070 | Li-Cor | 1/10,000 | WB | |
| IRDye® 680LT goat anti-rabbit IgG (H + L) | 926–68,071 | Li-Cor | 1/10,000 | WB |