Figure 2.

Upregulation of CD47 expression enhances resistance of cancer cells to T cell-mediated cytotoxicity

(a) Induction of cancer cell death (% Annexin-V^pos/PI^pos) in Retarg-1-decorated PC-3 or (b) PC-3.CD47KO cells when treated with HLA-matched CMV_pp65-specific T cells at indicated E:T cell ratios. (c) The percentage of Annexin-V positivity in PC-3 target cells ectopically overexpressing CD47 (PC-3.CD47^high, open circles and open boxes) versus empty vector-transduced PC-3 cells (PC-3.ev, solid black circles and solid black boxes) after treatment with CMV_pp65-specific T cells in the presence of Retarg-1. (d) IFNγ and (e) granzyme B levels in culture supernatants of (c) were measured by ELISA. Graphs represent mean ± SD. Experiments in A, B and C were evaluated by flow cytometry. Statistical analyses in A, B and C were performed using unpaired Student’s t‐test with Holm-Sidak post-hoc test.