Figure 9.

Administration of c2C7-DM1 ADC to mice bearing CAIX-positive MIA PaCa-2 tumors increases tumor cell death. (a) Representative images of tumor tissue sections from CAIX-positive MIA PaCa-2 PDAC xenografts stained for expression of CAIX. Scale bar, 100 μm; inset, 20 μm. (b-d) Mice bearing subcutaneous CAIX-positive MIA PaCa-2 PDAC xenografts were administered increasing doses of c2C7-DM1 (n = 8 mice/group). (b) Study timeline and tumor growth curve. ***P < .001; **P < .01; two-way ANOVA. (c) Tumor growth when control mice harboring tumors and administered vehicle reached the study endpoint. ***P < .001; *P < .05; two-way ANOVA. (d) Survival analysis of tumor-bearing mice administered 10 mg/kg c2C7-DM1, compared to mice given vehicle control. (e) Representative images of tumor tissue sections from CAIX-positive MIA PaCa-2 xenografts administered increasing doses of c2C7-DM1 and stained for cleaved caspase 3, a marker of apoptosis (black arrows). Scale bar, 50 μm; inset, 10 μm. (f) Quantification of cleaved caspase 3 (n = 5, each 5 fields). ***P < .001; *P < .05; two-way ANOVA. (g) Representative images of tumor tissue sections from CAIX-positive MIA PaCa-2 xenografts described in panel A stained with H&E to evaluate regions of necrosis. Boundaries between viable and necrotic tissue () are denoted with a black line. Scale bar, 100 μm. (h) Quantification of necrosis (n = 4, each 5 fields). ***P < .001; *P < .05; two-way ANOVA