Figure 4.

TNEA treatment increased TFEB expression and activated autophagy-lysosomal pathway in 5XFAD mice brains. (A) Representative western blots showed the levels of autophagy markers (LC3B and SQSTM1) in the TX-100 soluble and insoluble fractions from the prefrontal cortex (PFC) and hippocampus (HI) of mice brains. (B, C) Data were quantified as mean ± SEM (male, n = 6). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. 5XFAD group analyzed by Unpaired t test. (D) Representative western blots showed the levels of TFEB and lysosome markers (LAMP1 and CTSD) in the PFC and HI of mice brains. (E) Data were quantified as mean ± SEM (male, n = 6). *p < 0.05 and **p < 0.01 vs. 5XFAD group analyzed by Unpaired t test