Figure 10.

TFEB-induced lysosomal activation was required for memory improvement and APP/CTFs degradation induced by TNEA in 5XFAD mice. (A) Representative western blots showed the levels of full-length APP (Fl-APP) in the hippocampus of mice brains. (B) Data were quantified as mean ± SEM (n = 4) and analyzed by one-way ANOVA. **p < 0.01 and ***p < 0.001 vs. 5XFAD +TNEA group. (C) Morris water maze. Quantification of times spent in the target quadrant and number of platform crossed (mean ± SEM, n = 8–11) during probe trial. *p < 0.05 and **p < 0 .01 vs. 5XFAD+TNEA group analyzed by one‐way ANOVA. (D) Fear conditioning. The percentage of freezing in contextual and cued test was quantified (mean ± SEM, n = 8–11). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. 5XFAD+TNEA group analyzed by one‐way ANOVA. (E) Representative western blots showed the levels of full-length APP and carboxy-terminal fragments (CTFs) in the hippocampus of mice brains. (F) Relative levels of CTFs were quantified as mean ± SEM (male, n = 3). (G) Morris water maze. Quantification of number of platform crossed (mean ± SEM, n = 4–6) during probe trial. *p < 0.05 vs. sh-Scramble +TNEA group analyzed by one-way ANOVA