Table 2.
Summary of critical parameters for HDX-MS experimentation.80.
| |
Full screen |
Combo |
|||||
|---|---|---|---|---|---|---|---|
| Data Set | Control | 2C7 | 9B6 | 4A2 | G250 | Control | 4A2 + 9B6 |
| # of Peptides found across all samples | 75 | 77 | |||||
| Sequence coverage | 78% | 82% | |||||
| Average peptide length / Redundancy | 13.5/4.0 | 12.1/3.7 | |||||
| Repeatability (average SD, Da) | 0.07 | 0.03 | 0.04 | 0.05 | 0.05 | 0.04 | 0.03 |
| HDX reaction details | Phosphate buffered saline, pD = 6.9, RT | ||||||
| HDX time course (min) | 0.25, 1, 3, 10, 60 | ||||||
| Replicates (biological or technical) | 3 (technical) | ||||||
| Significant differences in HDX | Two-state student T-Test perfomed at each time point (>3 SD, p-value 0.02) | ||||||