Figure 1.
SOD1G93A mice have slow intestinal mobility, decreased rotarod test time and grip strength during ALS progression. (a) SOD1G93A mice significantly increased gut transit time starting at 2-month-old compared to WT mice. In age-matched WT and SOD1G93A mice, intestinal mobility was tested using Evans blue marker (5% Evans blue, 5% gum Arabic in drinking water). (Data are expressed as mean ± SD. n = 6, two-way ANOVA test, ***P < .001, adjusted by the Tukey method). (b) Starting at 2-month-old, SOD1G93A mice had significant reduced rotarod test time compared to WT mice. (Data are expressed as mean ± SD. n = 6, two-way ANOVA test, *P < .05, **P < .01, ***P < .001, adjusted by the Tukey method). (c) Forelimb grip strength in WT and SOD1G93A mice at different time points and (d) Hindlimb grip strength in WT and SOD1G93A mice at different time points. (Data are expressed as mean ± SD. n = 6, two-way ANOVA test, *P < .05, **P < .01, ***P < .001, adjusted by the Tukey method). (e) At the age of 2 months old, the expression of SMMHC protein started to decrease while the expression of GFAP protein started to increase in age matched SOD1G93A mice compared to WT mice, and (f) and (g) Quantification for the expression of SMMHC and GFAP proteins in different time points. (Data are expressed as mean ± SD. n = 6, Kruskal-Wallis test with pairwise comparisons using Wilcoxon rank sum exact test, *P < .05, ***P < .01, ***P < .001 adjusted by the FDR method)