Figure 7.
Increased aggregation of SOD1 mutant protein in human colonoids transfected with SOD1G93A-GFP plasmids and then colonized with SOD1G93A or WT mice feces. (a) Human colonoids were transfected with SOD1G93A-GFP plasmids after 48 hours and treated with feces from SOD1G93A/WT mice at different ages for 2 hours. After 2-hour treatment of mice feces, the phenotype of human colonoids, e.g., shape and buds did not change. Images are representative of experiments in triplicate. (b) Human SOD1 expression in human colonoids were transfected with SOD1G93A-GFP plasmids and then colonized with feces from SOD1G93A or WT mice. Images are representative of experiments in triplicate. (c) More SOD1G93A protein aggregations in the human colonoids after SOD1G93A-GFP plasmid transfection and 2 and 3-month-old SOD1G93A mice feces colonization. (Data are expressed as mean ± SD. n = 5, one-way ANOVA test, ***P < .001)