Figure 4.

TRPV4 is a potential biomarker for clinical diagnosis and treatment of glioma. (A-B) Correlation analysis of TRPV4 expression and tumor grade (A) or overall survival (B) in The Cancer Genome Atlas (TCGA) dataset. (C) mRNA expression levels of TRPV4 in GBM samples and paired normal control from the UALCAN database. (D) Correlation analysis between module eigengenes and common markers of glioma. WGCNA results demonstrated that TRPV4 gene was in the Red Module (ME Red). Red represents a positive correlation between ME and the specified glioma associated gene, whereas blue represents a negative correlation. Correlation coefficients were calculated using Pearson correlation with P-values in parentheses. (E) Correlation analysis between TRPV4 and glioma related molecular markers in the GSE50161 dataset. (F) Immunohistochemical determination of TRPV4 expression levels in a commercial tissue array of 126 glioma patients. (G) Representative immunohistochemistry images of the expression of TRPV4, SQSTM1, LC3-II in high-grade glioma patient samples. (H) Differential expression of TRPV4 protein in human GBM samples and normal control samples