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. 2021 Nov 22;10(1):2006529. doi: 10.1080/2162402X.2021.2006529

Figure 1.

Figure 1.

IL-2c enhances NK activation and maturation to treat orthotopic BC. Wild-type female mice were challenged orthotopically with 8 × 104 MB49 and treated with IL-2c or an isotype control every other day starting on day 7 for a total of 4 doses. (a-e) Mice were sacrificed on day 16 post challenge. Flow cytometric analysis of intratumoral bladder NK cell frequency (a), exhaustion (PD-1+) (b), activation (CD69+) (c), maturation (CD27−) (d), and CD69+ Eomes+ expression by CD27− NK cells (e). N = 6–8 pooled bladders/group. p, unpaired t test. NK cells in MB49 defined as CD45+ CD3− NK1.1+ cells. (f) Mouse survival in orthotopic MB49 challenge treated with IL-2c (as above) ± 200 µL α-asialo GM1 (asGM1). N = 7–9 mice/group. p, log-rank. Note: We previously reported data showing survival of isotype-treated mice bearing orthotopic MB49.20 Eomes, Eomesodermin. IL-2c, interleukin-2 complex. NK, Natural Killer. PD-1, Programmed Death Receptor 1