Figure 2.

Lipidated LC3 accumulates in thioglycolate-elicited peritoneal atg7^−/- macrophages in response to bafilomycin A₁. Representative LC3 immunoblotting in WT and atg7^−/- macrophages. (A) GAPDH and (B) ACTB/β-actin were used as loading controls. Cells were treated for (A) 2 h and (A and B) 14 h with bafilomycin A₁ (baf), (B) starved for 1 h in PBS (starv) or left untreated (C). Bars represent mean LC3-II:LC3-I ratios (+ SD) obtained from four individual mice. One-way ANOVA with Bonferroni correction were used to calculate differences between the different treatments; * p < 0.05, ** p < 0.01