Figure 2.

Proteins enriched on lipid droplets following lipophagy inhibition. (A) Schematic for human THP-1 macrophage lipid droplet (LD) isolation. (B) Western Blot analysis of LD and cytoplasmic fractions obtained by sucrose gradient ultracentrifugation. (C, D) Immunostaining of isolated LDs for PLIN2 and Ubiquitin (C), SQSTM1 and LC3 (D), and BODIPY 493/503 to label neutral lipids. (E) Enrichment of proteins at the surface of LDs in foam cells treated with chloroquine (CQ) relative to untreated (Ctrl), with the size of bubbles reflecting relative protein abundance. The number of putative ubiquitin-associated (UBA) and LC3-interacting (LIR) motifs identified in each protein is shown to the right. Scale bar: 1 μm