Figure 4.

Selective autophagy receptors (SARs) OPTN, NBR1 and SQSTM1 selectively localize to foam cell lipid droplets (LDs). (A-D) Immunostaining for OPTN (A), SQSTM1 (B), NBR1 (C) or CALCOCO2 (D) in agLDL-loaded mouse bone marrow-derived macrophage foam cells stained with BODIPY 493/503 to label LD neutral lipids, with areas of interest circled. At right, quantification of the percent of cellular LDs tagged with SARs in chloroquine-treated cells (CQ) as compared to control (Ctrl) is shown. Data are expressed as fold-change for the chloroquine treatment relative to untreated from one experiment representative of 3 independent experiments with similar results (mean ± s.e.m). **P< 0.005, ***P< 0.0005. Representative images are from CQ-treated cells. Scale bar: 5 μm