Figure 5.

Genetic ablation of selective lipophagy factors impairs lipophagy in yeast. (A) Representative growth curves of WT and atg8Δ strains in SC medium. (B) Activation of lipophagy during stationary phase shown by confocal fluorescence microscopy of Faa4-GFP lipid droplets (LDs) within Vph1-mRuby2 vacuoles. (C) Appearance of the GFP fragment of the Faa4-GFP fusion protein, indicating uptake and degradation of Faa4-GFP in the vacuole. (D) Faa4-GFP cleavage in various yeast stains. (E) LDs internalization into the vacuole at day 5 of stationary phase. (F) Lipophagy quantification. Scale bar: 5 μm