Table 1.
Recombinant DNA Constructs used in this study
| Plasmids | Protein | Notes |
|---|---|---|
| Flag-BLOC1S1 | BLOC1S1 | [3] |
| His-BLOC1S1 | BLOC1S1 | This work |
| pEX-PK-hLC3 (pHluorin-mKate2-LC3) | HsLC3B | pEX-PK-hLC3 was a gift from Isei Tanida (Addgene, 61,458) |
| mEos2-Lysosomes-20 (GFP-LAMP1) | LAMP1 | mEos2-Lysosomes-20 was a gift from Michael Davidson (Addgene, 57,397) |
| LAMP1-RFP | LAMP1 | LAMP1-RFP was a gift from Walther Mothes (Addgene, 1817) |
| pmRFP-LC3 | LC3B | pmRFP-LC3 was a gift from Tamotsu Yoshimori (Addgene, 21,075) |
| HA-KLC1 | KLC1 | [5] |
| GFP-ARL8B | ARL8B | [5] |
| GFP-KIF5B | KIF5B | [5] |
| MYC-PLEKHM2 | PLEKHM2 | [5] |
| KBS-LAMP1-GFP | KBS-LAMP1 | [5] |
| Flag-WHAMM | WHAMM | OriGene (CAT: MR223421) |
| pLJC5-TMEM192-3xHA (LysoIP) | TMEM192 | pLJC5-TMEM192-3xHA was a gift from David Sabatini (Addgene, 102,930) |