Figure 1.

TP53 is required for an acquired stress response to various stimuli. (A) Schematic of experimental procedures for preconditioned stress. WT and TP53 KO RPE-1 cells were preconditioned with or without total amino acids depletion (B), serine depletion (C), IR (2 Gy) (E) or HS (42°C) for 4 h (F), and then treated with low glucose (2 mM) media for 48 h. The proportions of dead cells (upper panels) and the relative cell numbers (lower panels) were determined at the indicated times as shown in (A). (D) WT RPE-1 cells were preconditioned with or without each single amino acid depletion, followed by incubation with low glucose (2 mM) media for 48 h. The proportions of dead cells (upper panel) and the relative cell numbers (middle panel) were determined at 48 h as shown in (A). Lysates from these cells at 48 h after treatment with low glucose (2 mM) media were subjected to immunoblotting using the indicated antibodies (lower panels). (G) WT and TP53 KO RPE-1 cells were preconditioned with or without glucose depletion and then treated with cysteine depletion for 48 h. The proportions of dead cells (upper panel) and the relative cell numbers (lower panel) were then determined at the indicated times. Data are presented as means±s.d. of three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons post hoc test was performed against a WT group mean (B-G). **P < 0.01, ***P < 0.001, ****P < 0.0001