Figure 2.

Lower amounts of ALs in activated CD8A T-cells from ART. (A-E) Ultrastructural analysis of purified CD8A T-cells from ART, EC and HIV^neg after 6 h of polyclonal activation (n = 3). (A) Representative ultrastructural micrographs from uninfected controls to differentiate AV (autophagic vacuoles; gray arrows) from ALs (autolysosomes; white arrows). (B) Ultrastructural micrographs in i. ART, ii. EC and iii. HIV^neg including strategic magnifications to appreciate the numbers of cellular vacuoles (X 8,000–17,000). Quantitative analysis of (C) the number and (D) percentages of positive CD8A T-cells for AV per 40 cells. (E) Number of ALs per 40 CD8A T-cells