FIG. 6.

E2F and p53 promoter reporter activity in TAg-transformed WT and ARF^−/− MEFs. The reporter constructs (A) 3xE2F-Luc and pCMV-β-Gal were cotransfected into the indicated stable TAg-transformed MEFs. Luciferase activity was normalized to β-galactosidase activity and expressed as relative luminometer units. The experiment was performed in triplicate; error bars indicate standard deviation. (B) Cyclin G promoter-luciferase reporter (left) and p21 promoter-luciferase promoter (right) activities were determined as described above.