Figure 5.

Effect of Ataxin-10 on MAPK/NF-κB signaling pathway in TNF-α-stimulated HUVECs. HA-Ataxin-10 or empty vector was transfected into HUVECs and then stimulated with TNF-α for the indicated times, and whole cell lysates were prepared and harvested for immunoblotting. Blots for activated p38 (phospho-p38 Thr180/Tyr182), activated p65 (phospho-p65S536), activated c-JUN (phospho-c-JUN Ser63), activated ERK (phospho-ERK T202/Y204), activated IκBα (phospho-IκBαSer32), activated IKKα/β (phospho-IKKα/βSer176/Ser177), and activated JNK (phospho-JNK T183/Y185) are shown. Internal loading is shown by antiactin or reprobing membranes with antibodies to total p38, p65, c-JUN, ERK1/2, IκBα, IKKα, IKKβ, and JNK immunoblotting. The bands of Western blotting were quantified by Gel-Pro Analyzer software, and the results were presented as fold changes at the right side of the bands.