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. 2021 Nov 11;9:737275. doi: 10.3389/fcell.2021.737275

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Copyright © 2021 Luo, Zhang, Wang, Zhao, Shen, Jia, Li, Zhang, Cai, Xiao, Bai, Liu, Wang, Zhang, Zhu, Zhou and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell viability of ASCs in SLF-3D and ULA-3D cultures at different time points. (A) Cell counting kit 8 (CKK-8) assay. Cell counting plot for ULA-3D and SLF-3D spheroids. Graphs show the means of three independent experiments, each performed in duplicate ±SE. n = 5; **p < 0.01, as compared with control (0 h) group. (B) Crystal violet stain of SLF-3D and ULA-3D ASC spheroids. (A) After 24, 48, 72, and 96 h, the spheroids were stained with crystal violet and photographed. Crystal violet staining: Cells were stained with crystal violet solution for 10 min. Three independent experiments were performed in duplicate, and representative results are shown. Scale bar, 100 μm.