FIGURE 6.

SLF-3D and ULA-3D spheroid cell viability was evaluated by flow cytometry. Viability of cells in the SLF-3D and ULA-3D spheroids. (A,B) Viability of ASCs as determined by flow cytometry measuring PI uptake and annexin V-FITC labeling. Representative log fluorescent dot plots and summary of the data are shown. As in the figure, 0, 3, 5ays, and 7 days indicate the number of days of cell culture. Cell populations were distinguished as live cells (PI-/annexinV-, lower left), or necrotic/dead/apoptotic cells (early apoptotic cells (PI−/Annexin V+), shown in the lower right; late apoptotic/dead cells (PI+/Annexin V+), shown in the upper right, and necrotic cells (PI+/Annexin V−), shown in the upper left. Three independent experiments were performed in duplicate, and representative results are shown. Data were acquired using BD Accuri C6 software.