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. 2021 Nov 11;9:777224. doi: 10.3389/fcell.2021.777224

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Copyright © 2021 Huang, Hong, Wu, Chen, Kong, Ruan, Teng and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Raman profiling of follicular fluid of PCOS and non-PCOS. (A) Averaged Raman spectra of follicular fluid of PCOS (n = 150) and non-PCOS (n = 150) patients. Shaded areas represent the standard deviations. (B) Mean-centered Raman spectra differences between PCOS and non-PCOS groups by subtracting means from all samples. (C) Principal component analysis (PCA) of all Raman Spectra to show the clustering of PCOS and non-PCOS groups. (D) Raman wavenumber loading plots of contributions along dimension 1 of the PCA, where grey boxes indicate the two Raman zones responsible for the most significant variances between the two groups by comparing the quantified spectral intensity values.