Figure 1.
The content of Tfh cells in peripheral blood and tumor tissues of patients with osteosarcoma. (a) Flow cytometry was used to detect CD4+CXCR5+Tfh cell content in peripheral blood of patients in the HC group and OS group. (b) Flow cytometry was used to detect CD4+CXCR5+Tfh cell content in tissues of the OS group. (c) Flow cytometry was used to detect the expression of PD-1 and CTLA-4 in CD4+CXCR5+Tfh cells in the peripheral blood of patients in the HC group and OS group (∗∗p < 0.01vs. HC group). (d) Flow cytometry was used to detect the expression of PD-1 and CTLA-4 in CD4+CXCR5+Tfh cells in the tissues of patients in the OS group (∗∗p < 0.01vs. normal group).