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. 2021 Nov 11;12:763288. doi: 10.3389/fmicb.2021.763288

FIGURE 3.

FIGURE 3

Effect of tet(X4)-bearing IncX1 plasmids encoding H-NS on the virulence phenotype of different recipient strains. (A–C) Survival of G. mellonella larvae infected by recipient strains and corresponding transformants, including E. coli TOP10 (A), K. pneumoniae ATCC700603 (B), or S. Enteritidis ATCC13076 (C). (D–F) Biofilm formation ability of three recipient strains and corresponding transformants, including E. coli TOP10 (D), K. pneumoniae ATCC700603 (E), or S. Enteritidis ATCC13076 (F). Biofilm mass was monitored using crystal violet staining. Data are representative of three biological replicates and shown as mean ± SD. p-values were determined using a non-parametric one-way ANOVA (*p < 0.05; ***p < 0.001; ****p < 0.0001; ns, not significant). (G–I) Swimming motility of three recipient strains and corresponding transformants. The mean diameters from three biological replicates were shown.