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. 2021 Nov 11;12:761795. doi: 10.3389/fimmu.2021.761795

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Copyright © 2021 Passos, Koh, Magalhães, Nunes, Gollob and Dutra

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of activation status of DN T-cell central (CM) and effector (EM) memory subpopulations, naive (N) and effector (EF) cells in indeterminate (IND, n = 6) and cardiac (CARD, n = 6) Chagas patients gauged by CD69 expression, before (MED) and after in vitro stimulation with live parasite (TRP). The graphs (A, B, top) represent the frequency of activated DN T-cell subpopulations in the different groups and under distinct conditions. The results are expressed as percentage in box plot, extending from the 25th to 75th percentile, with a horizontal line at the median with whiskers. Paired or unpaired t-tests were used to compare unstimulated and stimulated cultures or cultures between different groups of patients, respectively. Statistical significance is indicated in each graph. (A, B, bottom panels) Representative histograms of the expression of CD69 in the different DN T-cell subpopulations in IND and CARD. Clear curves show CD69 expresion in MED and gray curves show CD69 expression in TRP.