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Quantification of ROS, RNS and mitochondrial DNA in heart tissue. Lysates of left ventricular heart tissue were obtained from CCC (n = 40) and DCM (n = 31) patients and used for the quantification of (A) nitrite (nM) by chemiluminescence assay; (B) 3-nitrotyrosine by dot-blot and (C) copy number of the mitochondrial gene MT-ND1 by real time PCR. Red line: median; **p < 0.01; ***p < 0.001; Mann-Whitney test.
