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. 2021 Nov 11;15:752234. doi: 10.3389/fnana.2021.752234

FIGURE 2.

FIGURE 2

NeuN antibodies validation using confocal microscopy. (A–C) Representative confocal images of human brain slices stained using anti-NeuN antibody (ABN91; left): SWITCH (A), SHIELD (B), and CLARITY (C) samples, respectively. White arrows highlight three different stained neuronal bodies. On the right, autofluorescence signal of lipofuscin (white arrows). Excitation light, 568 nm; laser power, 1 mW. Objective lens, 60×; NA, 1.4. Scale bar = 50 μm. (D,E) Representative confocal images of SWITCH (D) and SHIELD (E) clarified human brain slices stained using ABN91, ARG 10712, and 26975-1-AP anti-NeuN antibodies. Scale bar = 50 μm.