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. 2021 May 25;35(12):3466–3481. doi: 10.1038/s41375-021-01282-6

Fig. 3. Multiplexed cytidine deamination reduces frequency of dsDNA break-mediated chromosomal translocations.

Fig. 3

A Schematic of TRBC locus within chromosome 7 q-arm and CD7 locus within chromosome 17 q-arm highlighted by red line. Four predicted chromosomal translocations generated following simultaneous dsDNA-mediated cleavage at TRBC and CD7 loci. B Gel electrophoresis of DNA products from either untransduced (UTD) or mixed 3CAR/7CAR co-cultures edited with SpCas9 or coBE3 mRNA following PCR amplification with TRBC Fwd - CD7 Fwd, TRBC Rev – CD7 Fwd, TRBC Rev – CD7 Rev and TRBC Fwd – CD7 Rev primer combinations. Positive bands detected at ~250 bp. Control bands are PCR amplicons from of synthesised fusions. C Histogram showing percentage of digital droplet PCR (ddPCR)-based quantification of four possible predicted translocations (T1-T4) in DNA from mixed 3CAR/7CAR co-cultures edited with SpCas9 or coBE3 mRNA.