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. 2021 Jun 12;35(12):3371–3382. doi: 10.1038/s41375-021-01315-0

Fig. 6. Glycolysis is important for the survival of TKI-persistent CML stem and progenitor cells.

Fig. 6

A Western blot for PKM2 using sensitive and IM-persistent K562 cell lysate. The bar plot represents density of the persistent protein bands relative to sensitive and normalized to the housekeeping protein, GAPDH. B Sensitive and IM-persistent K562 analyzed for apoptosis via flow cytometry post 3-day treatment with IM (1 µM), LLL12 (1 µM), or Shikonin (0.2 µM) alone, or in combination. C Colony-forming assay for 1000 LSK cells/mL sorted and plated from control, CML, and imatinib (400 mg/kg) treated CML mice. The plates were treated with IM (1 µM) or Shikonin (0.5 µM) alone, or in combination, incubated in hypoxia and colonies counted after 10 days. Data is represented as counts normalized to untreated. D Colony-forming assay for 1000 Lin-CD34+ BM cells/mL sorted and plated from 2 healthy individuals, 5 CML patients at diagnosis and 1 IM treated disease persistent patient. The plates were treated with Imatinib (1 µM) or Shikonin (0.5 µM) alone, or in combination, incubated in hypoxia and colonies counted after 10 days. Data are represented as counts normalized to untreated. The data are representative or a pool of mean ± SD from 2 to 3 independent experiments. Unpaired student t test or Two-way ANOVA was used to determine statistical significance with Tukey’s multiple comparison. p values < 0.05 were considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant.