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. 2021 Nov 4;9:749559. doi: 10.3389/fcell.2021.749559

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Copyright © 2021 Umer, Arévalo, Phadke, Lohanadan, Kirfel, Sons, Sofia, Witke and Schorle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunogold labeling of PFN3 in developing mouse spermatid during acrosome biogenesis. (A) cis- and trans-Golgi network; immunogold particles in cg and tg = black arrows. (B) Golgi phase; immunogold labeling highlighted in ag = circles, acp = white stars, and n = arrow head. (C) Cap phase; immunogold particles highlighted in av = asterisk. (D) Man immunogold labeling = white arrow heads. cg, cis-Golgi; tg, trans-Golgi; ag, acrosomal granule; n, nucleus; acp, acroplaxome; av, acrosomal vesicle. Scale bar = 500 nm. (A,B) are from the same image (ref.to Supplementary Figure 2A).