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. 2021 Nov 4;9:749559. doi: 10.3389/fcell.2021.749559

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Copyright © 2021 Umer, Arévalo, Phadke, Lohanadan, Kirfel, Sons, Sofia, Witke and Schorle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-immunoprecipitation using anti-PFN3 and anti-TRIM27 antibody on testis lysates. (A) Silver-stained SDS-PAGE; lane 1: input control (protein lysate), lane 2: negative control (only antibody), lane 3: IP using PFN3 antibody, lane 4: IP using PFN3 antibody, and lane 5: flow through. (B) Western blot showed PFN3 and TRIM27 proteins. Lane 1: input control. Lane 2: immunoblot of reciprocal IP for TRIM27 (band observed at ∼58 kDa) and PFN3 protein levels (band observed at ∼14 kDa) using anti-PFN3 and anti-TRIM27 antibodies, respectively. Lane 3: flow through. Lane 4: negative control.