Figure 5.

ADCC activity induced by EGCG against PR8 virus. PBS or 7 µg of HA antigens with or without adjuvant (30 μL of alum or 70, 350, or 700 μg of EGCG) were injected into mice thrice every 2 weeks. Sera were collected 2 weeks after the last vaccination. The sera were diluted 100× for the ADCC assay. Fold induction was defined as the increase in responses (number of times) compared to the negative control (reaction without mouse serum). Data are expressed as means (horizontal lines) and scatter plots. One-way ANOVA followed by Tukey’s multiple comparison test was conducted to compare three or more groups (*P < 0.05).