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SCORE binding assay of MIPD- and MIEA-derived peptide ligands to PSMα3. Real-time binding kinetics of L-MIPD27 and L-EA2 to D-PSMα3 as measured by SCORE. The mean intensities of four spots (EA2, MIPD27) and 2 spots (scrambled) with standard deviation are depicted. The association and dissociation phases are indicated; the dissociation kinetic starts shortly after induction of the washing step due to methodological reasons.
