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. 2021 Nov 12;11:651535. doi: 10.3389/fonc.2021.651535

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Copyright © 2021 Zhang, He, Shen, Wang, Liu and Jiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of miR-489-3p and its relationship with clinical prognosis of patients. (A) RNA-seq analysis the different expressed miRNAs associated with glycolysis. (B) q-RT-PCR analysis the upregulated miRNA in the exposure with glycolysis inhibitor 2-DG. (C, D) RNA in situ hybridization experiments showed the expression of miR-489-3p in paracancerous and cancerous tissues. The bar stands for 50 microns (E) qRT-PCR analysis of the relative expression of miR-489-3p in adjacent tissues and PC tissues. (F) qRT-PCR showed the relative expression of miR-489-3p in PC cell lines and pancreatic normal duct epithelial cells (HPDE). (G) Kaplan-Meier curve was divided into survival periods by miR-489-3p expression. Among them, patients were divided into high expression group (red) and low expression group (blue) by median expression of miR-489-3p. (*P < 0.05, **P < 0.01, ***P < 0.001).