TABLE 5.
Characteristics of the most prevalent serotypes among the STEC strains collected during this study
| Serotypea | Total no. of isolates | No. of isolates from:
|
Characteristics
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cattle | Beef | Cheese | Children | stx1b | stx2b | eaec | ehxAc | Sord | PGUAe | ||
| OX3:H2* | 13 | 11 | 1 | + | + | − | + | + | + | ||
| 1 | + | − | − | − | + | + | |||||
| O113:H21* | 12 | 6 | − | + | − | − | + | + | |||
| 4 | − | + | − | + | + | + | |||||
| 1 | + | + | − | + | + | + | |||||
| 1 | + | + | − | − | + | + | |||||
| O113:H4 | 9 | 6 | 2 | − | + | − | − | + | + | ||
| 1 | − | + | − | − | − | + | |||||
| OX3:H21* | 9 | 9 | − | + | − | − | + | + | |||
| O6:H10 | 8 | 6 | − | + | − | − | + | + | |||
| 1 | − | + | − | − | + | − | |||||
| 1 | + | − | − | − | + | + | |||||
| OX178:H19 | 8 | 6 | + | + | − | + | + | + | |||
| 2 | − | + | − | − | + | + | |||||
| O171:H2 | 7 | 5 | − | + | − | − | + | + | |||
| 2 | − | + | − | − | − | + | |||||
| O46:H38 | 7 | 7 | + | + | − | + | + | + | |||
| O172:H21 | 6 | 5 | + | + | − | − | − | + | |||
| 1 | − | + | − | − | − | + | |||||
| O22:H16 | 6 | 2 | 1 | − | + | − | + | + | + | ||
| 2 | + | + | − | − | + | + | |||||
| 1 | − | + | − | − | + | + | |||||
| O91:H10** | 6 | 6 | − | + | − | − | + | + | |||
| O91:H21** | 6 | 3 | 2 | − | + | − | + | + | + | ||
| 1 | + | + | − | + | + | + | |||||
| O22:H8* | 5 | 4 | − | + | − | − | + | + | |||
| 1 | − | + | − | + | + | + | |||||
| O109:H− | 4 | 2 | + | − | − | + | + | + | |||
| 1 | + | − | − | + | + | − | |||||
| 1 | + | + | − | + | + | + | |||||
| O74:H42 | 4 | 4 | + | + | − | + | + | + | |||
| O113:H− | 3 | 3 | − | + | − | − | + | + | |||
| O105:H18* | 3 | 3 | + | + | − | + | + | + | |||
| O112ac:H19 | 3 | 1 | 1 | − | + | − | − | + | + | ||
| 1 | + | + | − | + | + | + | |||||
| OX7:H16 | 3 | 3 | − | + | − | − | − | + | |||
| O8:H19 | 3 | 3 | + | + | − | + | + | + | |||
| O116:H28 | 2 | 2 | + | − | − | + | + | + | |||
| O132:H18 | 2 | 2 | − | + | − | − | + | + | |||
| O15:H16 | 2 | 2 | − | + | − | − | + | + | |||
| O22:H− | 2 | 1 | − | + | − | − | + | + | |||
| 1 | − | + | − | + | + | + | |||||
| O23:H15 | 2 | 2 | + | + | − | + | + | + | |||
| O40:H21 | 2 | 1 | + | + | − | + | + | + | |||
| 1 | − | + | − | + | + | + | |||||
| O49:H−* | 2 | 1 | − | + | + | + | − | − | |||
| 1 | + | − | + | + | − | + | |||||
| O96:H19 | 2 | 1 | + | + | − | − | − | + | |||
| 1 | − | + | − | + | − | + | |||||
*, serotypes known to be associated with severe disease (4, 21, 40); **, serotypes known to be associated with severe disease and isolated from HUS patients in the same area (5). Other serotypes isolated once: O1:H18, O1:H20, O2:H27, O2:H45, O6:H49, O20:H16, O39:H8, O39:H−, O77:H18, O84:H− O87:H16, O88:H21, O98:H−*, O102:H21, O103:H2*, O106:H42, O116:H21, O117:H7, O130:H43, O136:H12, O140:H32, O141:H−, O150:H−, O157:H7*, O159:H28, O163:H19*, “O”K84:H19, OX3:H43, OX3:H49, OX3:H−**, and OX177:H− from cattle; O25:H21, O54:H2, O75:H8, O128ab:H2*, O150:H8, O153:H8, O168:H8, and O172:H16 from beef samples; O8:H9, O15:H45, and O103:H14 from cheese samples; and O26:H21, O76:H19, and O141:H8 from children's stools.
Multiplex PCR results confirmed by hybridization for the stx genes. −, negative; +, positive.
Colony blot hybridization results for the eae and ehxA genes. −, negative; +, positive.
Sorbitol fermentation. −, negative; +, positive.
β-Glucuronidase production. −, negative; +, positive.