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. 2021 Nov 12;11:735180. doi: 10.3389/fonc.2021.735180

Figure 3.

Figure 3

MICAL2 promotes migration and invasion of glioma cells. (A–D) The effect of MICAL2 on cell migration was determined via wound-healing assay. Comparing with the NC group, the length of wound in MICAL2 knockdown group in U87 (p < 0.001) and U251 (p < 0.05) were significantly higher after 24 h of healing. (E, F) Transwell migration assay was used to detected cell invasion ability of GBM cells. Cell number of MICAL2 knockdown group were decreased in U87 (p < 0.05) and U251 (p < 0.05) cell lines compared with the NC group. (G, H) MICAL2, p-Smad2, and EMT relative protein (E-cadherin, N-cadherin, and vimentin) expression were detected via Western blotting. Data are shown as the means ± SEM of three experiments. Asterisk represents TGF-β-induced group comparing with the NC group; number sign represents TGF-β-induced and MICAL2-knockdown group comparing with TGF-β-induced group; *,#p < 0.05, **,## p < 0.01, ***,### p < 0.001.