Figure 5.

MICAL2 interacts with TGFRI. (A) Immunofluorescence was used to detect MICAL2 and TGFRI position in GBM cells. (B) Lysates of GBM cells were immunoprecipitated with anti-IgG, anti-MICAL2, or anti-TGFRI antibodies, respectively, and the products were analyzed by Western blotting with the indicated antibodies. (C) MICAL2, TGFRI, p-Smad2, and EMT relative protein (N-cadherin and vimentin) expression were detected via Western blotting. Data are shown as the means ± SEM of three experiments.Asterisk represents comparing with the TGF-β-induced group; ^** p < 0.01, ^*** p < 0.001.