Figure 3.

Bufalin inhibits the migration of CRC cells via the p53-PTEN/IGFBP3-mTOR axis. (A) HCT-116 or HT29 cells were treated with the sodium pump inhibitor bufalin (0, 10, 25, 50, 100, 200 nM) for 48 h and then harvested for Western blotting analysis. (B, C) HCT-116 cells were transfected with siRNAs targeting p53 in the presence or absence of bufalin (25 nM) for 48 h. A portion of cells were harvested for Western blotting analysis. The other portions were plated into Transwell plates overnight and then stained with crystal violet (0.5% w/v) for cell migration analysis. Scale bar, 100 μm. One-way ANOVA with Tukey’s multiple comparisons test was used. *p ≤ 0.05, **p ≤ 0.01.