Table 6.

Assigned peaks from the FTIR spectra of the metabolite produced by the Bacillus subtilis.

Run	Peak	Trans	Assignment
1	3998.26	56.19	O–H stretching vibration
2	3308.41	22.84	Strong N–H stretching vibration of H-bonding (due to water) and possible metal binding of amide groups in proteins
3	3199.74	14.94	C–H stretching vibration, of aliphatic Alkane
4	3021.58	32.71	C–H symmetric stretching vibration of the connection of the in CH2 group
5	2999.58	36.28	C–H symmetric stretching vibration of the connection of the in CH2 group
6	2982.55	38.49	C–H stretching vibration
7	2843.62	43.21	RCH = N=N stretching vibration
8	2228.71	52.70	C≡N stretching vibrations
9	1400.97	28.15	CH2 symmetric bending vibration
10	1200.04	31.57	C–O–C asymmetric stretching vibration
11	1026.48	32.20	C–O–C and C–C–O Stretching vibration due to the accumulation of polyester compounds (poly-3-hydroxybutyrate and probably phospholipids (PLs))
12	898.27	52.10	C–C Stretching vibration of amide group
13	800.00	48.76	CH bending vibration of alkenes
14	747.35	48.75	Rocking CH2 mode due to the accumulation of polyesters
15	632.07	46.21	C–O Stretching vibration
16	530.73	38.94	C–Cl C–Br, I Stretching vibrations

Trans. represent transmittance (%). Peak is cm⁻¹