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. 2021 Jul 15;42(12):2173–2180. doi: 10.1038/s41401-021-00722-4

Fig. 5. Immunohistochemical analysis of KCa1.1 in Ah-type BRNs of OVI, OVX female rats, and OVX treated with estrogen.

Fig. 5

Immunohistochemical co-localization of isolectin B4 (IB4) and KCa1.1 to verify the changes in the mean fluorescent density of identified myelinated Ah-type BRNs in tissue level of nodose ganglia from OVI, OVX, and OVX female rats treated with estrogen (E2) for 4 weeks after OVX procedure. IB4-positive neurons are presumably unmyelinated C-types. Both negative for IB4 and KCa1.1 are presumably myelinated A-types. IB4-negative but KCa1.1-positive neurons (indicated by white asterisks) are presumably myelinated Ah-types neurons.