Table 2.

The effects of G1, a selective agonist of GPR30, and G15, a selective antagonist of GPR30, on the repetitive discharge in identified myelinated Ah-type baroreceptor neurons (BRNs) isolated from adult ovary intact female rats.

Current-step depolarization (pA)	Control	G1 100 nM (%)	G1 + G15 3.0 μM
20	0.76 ± 0.33	4.51 ± 1.44 (~ 490)**	0.85 ± 0.57††
30	3.04 ± 0.86	9.44 ± 4.18 (~ 210)**	2.91 ± 1.06††
60	6.5 ± 2.3	12.5 ± 3.4 (~ 92)**	5.8 ± 3.8††
120	16.7 ± 4.4	20.2 ± 5.08 (~ 21)*	15.9 ± 4.6†

In this study, a single action potential (AP) is recorded routinely using a brief current pulse delivered to soma directly by recording electrode, which provides AP parameters and additional quantification for derivative change over the membrane potential to classify afferent fiber types upon the standard validation. On the same neuron, the current-step depolarization ranging from near threshold up to superthreshold is also delivered to soma before and after 100 nM G1 or G1 + 3.0 μM G15 to quantify the changes in repetitive discharge frequency at different levels of current-step depolarization. Additionally, G1-mediated increased percentage (%) of repetitive firing frequency was also estimated. Averaged data were expressed as mean ± SD, n = 5 complete recordings from 3 rats. *P < 0.05 and **P < 0.01 vs. control, ^†P < 0.05 and ^††P < 0^.01 vs. G1.